Chip Seq Library Preparation Kit

A minimum of 25 50 million read pairs are recommended for chip seq libraries.

Chip seq library preparation kit. Magna chip seq chip and ngs library preparation kit magna chip seq chromatin ip next generation sequencing library preparation kit containing all key reagents for chromatin ip next generation sequencing library preparation from genomic dna. The truseq chip kit has been designed for chip seq chromatin immunoprecipitation sequencing applications which allow for the determination and abundance of dna bound targets of interest across the genome. Read more about chip sequencing and the current kits and protocols for this next generation sequencing application. Microplex chemistry using stem loop adapters is specifically developed and optimized to generate dna libraries with high molecular complexity from the lowest input amounts.

A high performing fast and integrated workflow for sensitive applications such as human whole genome sequencing. Library prep kit selector. The nebnext chip seq library prep master mix set for illumina contains reagents for preparation of libraries for next generation sequencing on the illumina platform from 10 ng of chip dna in a standard workflow. Diagenode s microplex library preparation kits have been extensively validated for chip seq samples.

Offering the proven data quality and ease of use of truseq dna sequencing they provide a streamlined chip seq library preparation workflow that leverages reagent master mixes to minimize pipetting and reduce total assay time. Chip seq dna sample prep kit support. Generated libraries are compatible with single end or paired end sequencing. Simple hands on time 10 min.

The average insert size of a chip seq library constructed with the nebnext chip seq library prep kit is approximately 300 to 500 bp. Chip seq dna sample prep kit support resources. See genohub s up to date list of available illumina chip seq library prep services or if you re doing your own library preparation see the. Each of these components must pass rigorous quality control standards and are lot controlled both individually and as a set of reagents.

How many sequence reads are recommended for chip seq analysis. Truseq chip library preparation kits are compatible with all illumina sequencers. The chip seq library prep reagent set for illumina contains enzymes and buffers that are ideally suited for sample preparation for next generation sequencing and for preparation of expression libraries. Find msds or sds a coa data sheets and more information.

Please note that adaptors and primers are not included in the kit and are available separately. Chip seq library prep fast total time 1 5 hrs.